A Bees side made up of mainly reserves and trialists lost 3-1 at Sutton United.
In the second half, Warren McBean doubled Sutton's lead in the 67th minute. Two minutes later, Charlie Ide latched on to a through ball from trialist Jason Karaca to net Bees' goal.
Sutton wrapped up the game in the 84th minute when McBean scored again.
Brentford Squad
Masters, Dark, Tillen, Carder-Andrews, Heywood, Charles, Kieron Murtagh (Trialist) (sub Mousinho 81), Jason Karaca (Trialist), Montague, Ide, Peters
Att: 427